ALAS2 Monoclonal antibody, PBS Only (Capture)

ALAS2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1H1

Cat no : 68332-1-PBS

Synonyms

ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial

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Validation Data Gallery

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  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50624-1

  Cytometric bead array standard curve of MP50624-1, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-2-PBS. Standard:Ag13524. Range: 6.25-100 ng/mL.

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  Cytometric bead array standard curve of MP50624-2

  Cytometric bead array standard curve of MP50624-2, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-3-PBS. Standard:Ag13524. Range: 3.125-100 ng/mL.

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Product Information

68332-1-PBS targets ALAS2 as part of a matched antibody pair:

MP50624-1: 68332-1-PBS capture and 68332-2-PBS detection (validated in Cytometric bead array)

MP50624-2: 68332-1-PBS capture and 68332-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALAS2 fusion protein Ag13524
• Full Name: aminolevulinate, delta-, synthase 2
• Calculated Molecular Weight: 587 aa, 65 kDa
• Observed Molecular Weight: 60~65 kDa
• GenBank Accession Number: BC030230
• Gene Symbol: ALAS2
• Gene ID (NCBI): 212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.