AKTIP Polyclonal antibody

AKTIP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14860-1-AP

Synonyms

FTS, Ft1, AKT-interacting protein

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Validation Data Gallery

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  WB analysis of HepG2 using 14860-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14860-1-AP (AKTIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14860-1-AP

  IP result of anti-AKTIP (IP:14860-1-AP, 4ug; Detection:14860-1-AP 1:300) with HepG2 cells lysate 1680 ug.

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  IF Staining of SH-SY5Y using 14860-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14860-1-AP (AKTIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

14860-1-AP targets AKTIP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKTIP fusion protein Ag6662
• Full Name: AKT interacting protein
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001134
• Gene Symbol: AKTIP
• Gene ID (NCBI): 64400
• RRID: AB_2878087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Rep; AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance; Authors - Angel S N Ng ; KD Validated