Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 80816-1-RR
WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells.
IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug.
IHC staining of human ovary tumor using 80816-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 80816-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of Jurkat using 80816-1-RR
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected in
Jurkat cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.
Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 80816-1-RR
WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells.
IHC Figures
IHC staining of human ovary tumor using 80816-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 80816-1-RR
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HEK-293 using 80816-1-RR
IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug.
FC (INTRA) Figures
FC experiment of Jurkat using 80816-1-RR
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
AKT1-Specific Recombinant antibody
Catalog Number
80816-1-RR
Citations
3
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
