AKT Polyclonal antibody

• Featured Product
• KD/KO Validated

AKT Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 51077-1-AP

Synonyms

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

---

Validation Data Gallery

• 

  WB analysis using 51077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51077-1-AP (AKT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 51077-1-AP

  WB result of AKT1 antibody (51077-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells.

• 

  WB analysis using 51077-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51077-1-AP (AKT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 51077-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51077-1-AP (AKT1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 51077-1-AP

  Immunofluorescent analysis of HeLa cells using 51077-1-AP (AKT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, MCF-7 cells, A549 cells, COLO 320 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 1 publications below

Product Information

51077-1-AP targets AKT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56-62 kDa
• GenBank Accession Number: BC084538
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• RRID: AB_2242475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Publications

Species	Application	Title
human	WB	Elife; Binding blockade between TLN1 and integrin β1 represses triple-negative breast cancer.; Authors - Yixiao Zhang
human	WB	Front Oncol; Low Expression Levels of SLC22A12 Indicates a Poor Prognosis and Progresses Clear Cell Renal Cell Carcinoma.; Authors - Jiaju Xu
human	WB	Biomed Pharmacother; α-Terthienyl induces prostate cancer cell death through inhibiting androgen receptor expression.; Authors - Xia Gan
mouse	WB	Sci Rep; Aronia melanocarpa polysaccharide ameliorates inflammation and aging in mice by modulating the AMPK/SIRT1/NF-κB signaling pathway and gut microbiota; Authors - Yingchun Zhao
rat	WB	Front Cell Neurosci; Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model.; Authors - Lin-Yan Huang
human	WB	J Cell Biochem; Combined detection of estrogen and tumor markers is an important reference factor in the diagnosis and prognosis of lung cancer.; Authors - Yuquan Bai