AKT Substrate Expanded Antibody Kit

Host

Rabbit/Mouse

Reactivity

Target Dependent

Applications

Target Dependent

Conjugate

Unconjugated

Cat no : PK30022



Product Information

The AKT Substrate Expanded Antibody Kit provides a cost-effective tool for studying a broad range of proteins that are phosphorylated by AKT. Perfect for signal transduction researchers starting a new project, screening multiple prospective targets, or those who simply require less volume. 

The AKT Substrate Expanded Kit contains 10 antibodies against downstream phosphorylation targets of AKT.


AntigenCatalog No.Host, clonalityTested ReactivityApplicationsVolume
Phospho-GSK3B
(Ser9)
67558-1-Ig            Mouse monoclonalH      WB, IHC, IF            20 uL
Phospho-mTOR
(Ser2448)
80596-1-RR            Rabbit monoclonal            H, RWB, IF           20 uL
Phospho-TSC2
(Thr1462)
80698-1-RR            Rabbit monoclonalHWB          20 uL
Phospho-TSC2
(Ser939)
81654-1-RR            Rabbit monoclonal            H WB         20 uL
Phospho-FOXO1
(Ser319)
28757-1-AP
           
Rabbit polyclonal            H  WB      20 uL            
Phospho-CREB1
(Ser133)
81871-1-RR
           
Rabbit monoclonal            HWB, IHC           20 uL            
Phospho-Caspase 9
(Ser196)
80346-1-RR
           
Rabbit monoclonal            H, M   WB          20 uL            
Phospho-XIAP
(Ser87)
28791-1-AP
           
Rabbit polyclonal            H, M, R   WB            20 uL            
Phospho-ASK1
(Ser83)
28847-1-AP
           
Rabbit polyclonal            HWB            20 uL            
Phospho-AKT
(Ser473)
80455-1-RR
           
Rabbit monoclonal            H       WB          20 uL            


Also see our 'AKT Substrate Essentials Antibody Kit' on the following page  https://www.ptglab.com/products/AKT-Substrate-Essentials-Antibody-Kit-PK30021.htm

Storage

Store at -20°C. Stable for one year from the date of receipt. 

Background Information

Upon its activation, AKT phosphorylates a number of different substrates, resulting in the activation or inactivation of downstream cellular functions. The phosphorylation of GSK3B at Ser9, inhibits its activity and subsequent glycogen synthesis. Meanwhile, AKT-mediated phosphorylation of mTOR at Ser2448 results in its activation and formation of the mTORC1 complex. TSC2, an inhibitor of mTORC1 activation, is blocked through phosphorylation at Thr1462 and Ser939 residues. FOXO1, a transcription factor invovled in cell cycle arrest and apoptosis, is inhibited by AKT through phosphorylation at Ser319, which leads to its nuclear export. Pro-survival functions are also promoted through inhibiting ASK1 activity via Ser83 phosphorylation and XIAP through Ser87 phosphorylation. Finally, AKT can help to trigger the caspase cascade by phosphorylating Caspase 9 at Ser196. 

Standard Protocols

Click   here    to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30022, AKT Substrate Expanded Antibody Kit, Proteintech, IL, USA