AKT1/3 Monoclonal antibody

• Featured Product
• KD/KO Validated

AKT1/3 Monoclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

57

CloneNo.

3G5E11

Cat no : 60203-1-Ig

Synonyms

AKT, AKT 1, AKT1, AKT1/3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 60203-1-Ig

  WB result of AKT1/3 antibody (60203-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1/3 transfected MCF-7 cells.

• 

  WB analysis of HeLa using 60203-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 60203-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60203-1-Ig

  IP result of anti-AKT1/3 (IP:60203-1-Ig, 5ug; Detection:60203-1-Ig 1:600) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human cervical cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, rat liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 56 publications below
• IF: See 1 publications below

Product Information

The immunogen of 60203-1-Ig is AKT1/3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, huamn
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AKT1/3 fusion protein Ag16695
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56-62 kDa
• GenBank Accession Number: BC000479
• Gene Symbol: AKT1
• Gene ID (NCBI): 207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
human	WB	Theranostics; Hypoxia-induced TGFBI maintains glioma stem cells by stabilizing EphA2; Authors - Zirong Chen
human	WB	J Exp Clin Cancer Res; Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization; Authors - Yuanshan Yao
human	WB	Sci Total Environ; Effects of triclosan exposure on stem cells from human exfoliated deciduous teeth (SHED) fate; Authors - Shiwen Deng
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
human	WB	Cancer Lett; Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.; Authors - Shan-Shan Guo