AKT1/3 Monoclonal antibody

• Featured Product
• KD/KO Validated

AKT1/3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Publications

45

CloneNo.

3G5E11

Cat no : 60203-1-Ig

Synonyms

AKT, AKT 1, AKT1, AKT1/3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 60203-1-Ig

  WB result of AKT1/3 antibody (60203-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1/3 transfected MCF-7 cells.

• 

  WB analysis of HeLa using 60203-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 60203-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60203-1-Ig

  IP Result of anti-AKT1/3 (IP:60203-1-Ig, 5ug; Detection:60203-1-Ig 1:600) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens).

• 

  IF Staining of MCF-7 using 60203-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using AKT1 antibody 60203-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 60203-1-Ig

  1X10^6 MCF-7 cells were stained with 0.2ug AKT1/3 antibody (60203-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

---

Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, rat liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 44 publications below
• IF: See 1 publications below

Product Information

The immunogen of 60203-1-Ig is AKT1/3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, huamn
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AKT1/3 fusion protein Ag16695
• Full Name: v-akt murine thymoma viral oncogene homolog 1
• Calculated molecular weight: 56 kDa
• Observed molecular weight: 56-62 kDa
• GenBank accession number: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.

Publications

Species	Application	Title
rat	WB	Mol Med Rep; Function of BRD4 in the pathogenesis of high glucose‑induced cardiac hypertrophy.; Authors - Qian Wang
human	WB	J Immunol; Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice.; Authors - Zhiping Ren
mouse	WB	Front Physiol; Autophagy Protects the Blood-Brain Barrier Through Regulating the Dynamic of Claudin-5 in Short-Term Starvation.; Authors - Zhenguo Yang
rat	WB	Biol Pharm Bull; Astragaloside IV regulates the PI3K/Akt/HO-1 signaling pathway and inhibits H9c2 cardiomyocyte injury induced by hypoxia-reoxygenation.; Authors - Ping Yang
mouse	WB	Behav Brain Res; Resveratrol synergizes with low doses of L-DOPA to improve MPTP-induced Parkinson disease in mice.; Authors - Qianqian Liu
mouse	WB	J Immunol; Leptin Promotes Monosodium Urate Crystal-Induced Inflammation in Human and Murine Models of Gout.; Authors - Yiyun Yu