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  • KD/KO Validated

AKT Polyclonal antibody

AKT Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 10176-2-AP

Synonyms

AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells, C6 cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse brain tissue
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10176-2-AP targets AKT in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, zebra finches, fish, Zebrafish, hamster, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT fusion protein Ag0213
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56-62 kDa
GenBank Accession NumberBC000479
Gene Symbol AKT1
Gene ID (NCBI) 207
RRIDAB_2224574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Protocols

Product Specific Protocols
WB protocol for AKT antibody 10176-2-APDownload protocol
IHC protocol for AKT antibody 10176-2-APDownload protocol
IF protocol for AKT antibody 10176-2-APDownload protocol
IP protocol for AKT antibody 10176-2-APDownload protocol
FC protocol for AKT antibody 10176-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

RAB31 marks and controls an ESCRT-independent exosome pathway.

Authors - Denghui Wei
mouseWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
humanWB

Nat Commun

Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk

Authors - Pingting Ying
humanWB

Sci Adv

Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.

Authors - Patrick Han
mouseWB

Acta Pharm Sin B

Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment

Authors - Lin Hou
humanWB

Brain

MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.

Authors - Bing-Lin Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hyopil (Verified Customer) (08-23-2021)

Worked well with western blotting

  • Applications: Western Blot
  • Cell Tissue Type: HAP1
FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: NSC34