AKR7A3 Polyclonal antibody

AKR7A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13209-1-AP

Synonyms

EC:1.-.-.-, AFB1-AR 2, AFB1 AR 2, AFB1 aldehyde reductase 2, AFAR2

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Validation Data Gallery

• 

  WB analysis of human liver using 13209-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13209-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13209-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13209-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human liver tissue, HepG2 cells, human brain tissue, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13209-1-AP targets AKR7A3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR7A3 fusion protein Ag3991
• Full Name: aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)
• Calculated Molecular Weight: 331 aa, 37 kDa
• Observed Molecular Weight: 37 kDa and 55-60 kDa
• GenBank Accession Number: BC025709
• Gene Symbol: AKR7A3
• Gene ID (NCBI): 22977
• RRID: AB_2224549
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKR7A3 belongs to the aldo‐keto reductase (AKR) superfamily, whose primary role is to reduce aldehydes and ketones to generate primary and secondary alcohols, respectively. These enzymes have been shown to play crucial roles in drug metabolism, carcinogen metabolism, and cellular metabolism. Growing evidence suggests that AKR7A3 can play an essential role in the occurrence of cancers, including breast and liver cancers. (PMID: 36951402)

Publications

Species	Application	Title
rat	WB	Toxicol Sci; Generation of A New Model Rat: Nrf2 Knockout Rats Are Sensitive to Aflatoxin B1 Toxicity.; Authors - Keiko Taguchi