AKR1C3 Recombinant antibody

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  WB analysis using 84872-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84872-5-RR (AKR1C3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

AKR1C3 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 84872-5-RR

Clone No.242222A11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

3-alpha-hydroxysteroid dehydrogenase type 2, 3-alpha-HSD type II, brain, 3-alpha-HSD type 2, 242222A11, 17-beta-hydroxysteroid dehydrogenase type 5

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Tested Applications

• Positive WB detected in: K-562 cells, A549 cells, HepG2 cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84872-5-RR targets AKR1C3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AKR1C3 fusion protein Ag1674
• Full Name: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Calculated Molecular Weight: 323 aa, 37 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC019230
• Gene Symbol: AKR1C3
• Gene ID (NCBI): 8644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42330
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.