AKR1B10 Monoclonal antibody

AKR1B10 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5A4G5

Cat no : 68327-1-Ig

Synonyms

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HIS, HSI, SI reductase, Small intestine reductase

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Validation Data Gallery

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  WB analysis using 68327-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat stomach using 68327-1-Ig

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig small intestine using 68327-1-Ig

  pig small intestine tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 68327-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1B10 antibody (68327-1-Ig, Clone: 5A4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, rat stomach tissue, pig small intestine tissue, A549 cells, pig stomach tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68327-1-Ig targets AKR1B10 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat samples.

• Tested Reactivity: Human, Pig, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AKR1B10 fusion protein Ag24406
• Full Name: aldo-keto reductase family 1, member B10 (aldose reductase)
• Calculated Molecular Weight: 316 aa, 36 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC008837
• Gene Symbol: AKR1B10
• Gene ID (NCBI): 57016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldo-keto reductase family 1 member B10 (AKR1B10), which belongs to the AKR superfamily, consists of 316 amino acids and its gene is located on chromosome 7q33. AKR1B10 was initially identified in liver cancer. It is highly expressed in the small intestine, colon, and adrenal gland.