AKR1B1 Monoclonal antibody

• Featured Product
• KD/KO Validated

AKR1B1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2F11G5

Cat no : 67498-1-Ig

Synonyms

ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR

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Validation Data Gallery

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  WB analysis of HEK-293 using 67498-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67498-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67498-1-Ig

  WB result of AKR1B1 antibody (67498-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected HepG2 cells.

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  WB analysis of HepG2 using 67498-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67498-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67498-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67498-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67498-1-Ig targets AKR1B1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AKR1B1 fusion protein Ag7571
• Full Name: aldo-keto reductase family 1, member B1 (aldose reductase)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000260
• Gene Symbol: AKR1B1
• Gene ID (NCBI): 231
• RRID: AB_2882722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification; Authors - Wei Zhou
mouse	WB	Pharmacol Res; Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy.; Authors - Liman Luo