AKR1B1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

AKR1B1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15439-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A431 using 15439-1-AP (same clone as 15439-1-PBS)

  WB result of AKR1B1 antibody (15439-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected A431 cells. This data was developed using the same antibody clone with 15439-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15439-1-AP (same clone as 15439-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 15439-1-AP (same clone as 15439-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 15439-1-AP (same clone as 15439-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 15439-1-AP (same clone as 15439-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15439-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15439-1-AP (same clone as 15439-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15439-1-AP (AKR1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15439-1-PBS in a different storage buffer formulation.

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Product Information

15439-1-PBS targets AKR1B1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1B1 fusion protein Ag7142
• Full Name: aldo-keto reductase family 1, member B1 (aldose reductase)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000260
• Gene Symbol: AKR1B1
• Gene ID (NCBI): 231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.