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  • Featured Product
  • KD/KO Validated

AKR1B1 Polyclonal antibody

AKR1B1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15439-1-AP

Synonyms

EC:1.1.1.21, AR, ALR2, ALDR1, Aldo-keto reductase family 1 member B1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using AKR1B1 Polyclonal antibody (15439-1-AP)

    WB analysis of A431 using 15439-1-AP

    WB result of AKR1B1 antibody (15439-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using AKR1B1 Polyclonal antibody (15439-1-AP)

    WB analysis using 15439-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AKR1B1 Polyclonal antibody (15439-1-AP)

    WB analysis of L02 using 15439-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using AKR1B1 Polyclonal antibody (15439-1-AP)

    WB analysis of human liver using 15439-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AKR1B1 Polyclonal antibody (15439-1-AP)

    WB analysis of L02 using 15439-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AKR1B1 Polyclonal antibody (15439-1-AP)

    IF Staining of HepG2 using 15439-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15439-1-AP (AKR1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, L02 cells, human liver tissue, BxPC-3 cells, HepG2 cells, human placenta tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15439-1-AP targets AKR1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1B1 fusion protein Ag7142
Full Name aldo-keto reductase family 1, member B1 (aldose reductase)
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000260
Gene Symbol AKR1B1
Gene ID (NCBI) 231
RRIDAB_2224154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKR1B1 antibody 15439-1-APDownload protocol
IF protocol for AKR1B1 antibody 15439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Oxid Med Cell Longev

WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.

Authors - Xiaoyu Zhou
View Article
humanWB

Biochem Pharmacol

Regulation on SIRT1-PGC-1α/Nrf2 pathway together with selective inhibition of aldose reductase makes compound hr5F a potential agent for the treatment of diabetic complications.

Authors - Zhihua Wang
View Article
ratWB

Biomed Pharmacother

Systematic investigation of the pharmacological mechanism for renal protection by the leaves of Eucommia ulmoides Oliver using UPLC-Q-TOF/MS combined with network pharmacology analysis.

Authors - Qi Huang
View Article
ratWB

Toxicol Appl Pharmacol

Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.

Authors - Zhanjun Ma
View Article
humanWB

BMC Nephrol

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

Authors - Yang Lu
View Article
humanWB

Fertil Steril

Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation.

Authors - Valentina Murdica
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Parijat (Verified Customer) (01-12-2024)

Good antibody for studying hypertonic stress response.

  • Applications: Flow Cytometry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: IMCD3 cells