AKR1B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

AKR1B1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 15439-1-AP

Synonyms

EC:1.1.1.21, AR, ALR2, ALDR1, Aldo-keto reductase family 1 member B1

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Validation Data Gallery

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  WB analysis of A431 using 15439-1-AP

  WB result of AKR1B1 antibody (15439-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected A431 cells.

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  WB analysis using 15439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15439-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15439-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15439-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15439-1-AP (AKR1B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15439-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15439-1-AP (AKR1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, L02 cells, human liver tissue, BxPC-3 cells, HepG2 cells, human placenta tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 1 publications below

Product Information

15439-1-AP targets AKR1B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1B1 fusion protein Ag7142
• Full Name: aldo-keto reductase family 1, member B1 (aldose reductase)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000260
• Gene Symbol: AKR1B1
• Gene ID (NCBI): 231
• RRID: AB_2224154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.; Authors - Xiaoyu Zhou
human	WB	Biochem Pharmacol; Regulation on SIRT1-PGC-1α/Nrf2 pathway together with selective inhibition of aldose reductase makes compound hr5F a potential agent for the treatment of diabetic complications.; Authors - Zhihua Wang
rat	WB	Biomed Pharmacother; Systematic investigation of the pharmacological mechanism for renal protection by the leaves of Eucommia ulmoides Oliver using UPLC-Q-TOF/MS combined with network pharmacology analysis.; Authors - Qi Huang
rat	WB	Toxicol Appl Pharmacol; Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.; Authors - Zhanjun Ma
human	WB	BMC Nephrol; Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.; Authors - Yang Lu
human	WB	Fertil Steril; Seminal plasma of men with severe asthenozoospermia contain exosomes that affect spermatozoa motility and capacitation.; Authors - Valentina Murdica

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parijat (Verified Customer) (01-12-2024)

Good antibody for studying hypertonic stress response.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: IMCD3 cells