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AKAP3 Recombinant antibody, PBS Only

AKAP3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230278C3

Cat no : 82986-1-PBS

Synonyms

AKAP-3, AKAP110, AKAP 3, AKAP 110, A kinase anchor protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKAP3 Recombinant antibody (82986-1-RR)

    WB analysis using 82986-1-RR (same clone as 82986-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82986-1-RR (AKAP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82986-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82986-1-RR

    Affinity and Kinetic Characterization of 82986-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82986-1-RR against Human AKAP3 were performed. The affinity constant is below 1 pM.

Product Information

82986-1-PBS targets AKAP3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AKAP3 fusion protein Ag33655
Full Name A kinase (PRKA) anchor protein 3
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.