AKAP3 Monoclonal antibody
AKAP3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3C11H9
Cat no : 66255-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human testis tissue |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66255-1-Ig targets AKAP3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | AKAP3 fusion protein Ag4885 |
Full Name | A kinase (PRKA) anchor protein 3 |
Calculated Molecular Weight | 95 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC047535 |
Gene Symbol | AKAP3 |
Gene ID (NCBI) | 10566 |
RRID | AB_2881643 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.
Protocols
Product Specific Protocols | |
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WB protocol for AKAP3 antibody 66255-1-Ig | Download protocol |
IHC protocol for AKAP3 antibody 66255-1-Ig | Download protocol |
IF protocol for AKAP3 antibody 66255-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |