AKAP14 Polyclonal antibody

AKAP14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14622-1-AP

Synonyms

A kinase anchor protein 14, A kinase anchor protein 28 kDa, AKAP 14, AKAP 28, AKAP14, AKAP28

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Validation Data Gallery

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  WB analysis of Jurkat using 14622-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14622-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 14622-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, human testis tissue
• Positive IHC detected in: human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14622-1-AP targets AKAP14 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKAP14 fusion protein Ag6255
• Full Name: A kinase (PRKA) anchor protein 14
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC066357
• Gene Symbol: AKAP14
• Gene ID (NCBI): 158798
• RRID: AB_2225760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.