AK5 Polyclonal antibody

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  WB analysis using 12510-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12510-2-AP (AK5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 12510-2-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12510-2-AP (AK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12510-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12510-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12510-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12510-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12510-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12510-2-AP (AK5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12510-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12510-2-AP (AK5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

AK5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 12510-2-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

KAD5, EC:2.7.4.3, ATP-AMP transphosphorylase 5, AK 5

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse cerebellum tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

12510-2-AP targets AK5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AK5 fusion protein Ag3203
• Full Name: adenylate kinase 5
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC012467
• Gene Symbol: AK5
• Gene ID (NCBI): 26289
• RRID: AB_2305376
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6K8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human, rat	WB,IF	J Neurol Sci; Down-regulation of adenylate kinase 5 in temporal lobe epilepsy patients and rat model.; Authors - Yujie Lai