AK2 Monoclonal antibody, PBS Only (Capture)

• Featured Product
• KD/KO Validated

AK2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat no : 66127-1-PBS

Synonyms

ATP:AMP phosphotransferase, ADK2, Adenylate monophosphate kinase, Adenylate kinase 2, mitochondrial, N-terminally processed, adenylate kinase 2

---

Validation Data Gallery

• 

  WB analysis using 66127-1-Ig (same clone as 66127-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

  WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66127-1-Ig (same clone as 66127-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AK2 antibody (66127-1-Ig, Clone: 3H2C10 ) at dilution of 1:5024 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

  Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50407-1

  Cytometric bead array standard curve of MP50407-1, AK2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66127-1-PBS. Detection antibody: 66127-2-PBS. Standard:Ag17911. Range: 0.195-50 ng/mL.

---

Product Information

66127-1-PBS targets AK2 as part of a matched antibody pair:

MP50407-1: 66127-1-PBS capture and 66127-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AK2 fusion protein Ag17911
• Full Name: adenylate kinase 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC009405
• Gene Symbol: AK2
• Gene ID (NCBI): 204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.