AIFM2/ FSP1 Monoclonal antibody, PBS Only

AIFM2/ FSP1 Monoclonal Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B2

Cat no : 68049-1-PBS

Synonyms

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

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Validation Data Gallery

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  WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 68049-1-Ig (same clone as 68049-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 68049-1-Ig (same clone as 68049-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68049-1-Ig (same clone as 68049-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68049-1-Ig (same clone as 68049-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

  IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

• 

  FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68049-1-PBS targets AIFM2/ FSP1 in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AIFM2/ FSP1 fusion protein Ag30516
• Full Name: apoptosis-inducing factor, mitochondrion-associated, 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC023601
• Gene Symbol: AIFM2
• Gene ID (NCBI): 84883
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.