AIDA Polyclonal antibody

• Featured Product
• KD/KO Validated

AIDA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 23724-1-AP

Synonyms

AIDA, C1orf80, RP11 378J18.7

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 23724-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23724-1-AP (AIDA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23724-1-AP

  IP result of anti-AIDA (IP:23724-1-AP, 3ug; Detection:23724-1-AP 1:600) with HeLa cells lysate 4000ug.

• 

  IHC staining of mouse skeletal muscle using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 0x lens).

• 

  IHC staining of mouse skeletal muscle using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 23724-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23724-1-AP (AIDA antibody) at dilution of 1:100 (under 10x lens).

---

Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 23724-1-AP is AIDA Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AIDA fusion protein Ag20588
• Full Name: axin interactor, dorsalization associated
• Calculated Molecular Weight: 306 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC015535
• Gene Symbol: AIDA
• Gene ID (NCBI): 64853
• RRID: AB_2879311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

Publications

Species	Application	Title
mouse	IP	Nat Cell Biol; AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.; Authors - Meng Shi ; KO Validated
mouse	WB,IF	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

David (Verified Customer) (02-22-2022)

Unfortunately, the antibody-conjugated magnetic beads did not show a strong signal for the Luminex-based assay.

• Applications: Other
• Primary Antibody Dilution: 10ug/mL
• Cell Tissue Type: Human