AHR Recombinant antibody

AHR Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240325G9

Cat no : 83369-6-RR

Synonyms

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

---

Validation Data Gallery

• 

  IF Staining of HepG2 using 83369-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 83369-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 83369-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83369-6-RR is AHR Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AHR fusion protein Ag12193
• Full Name: aryl hydrocarbon receptor
• Calculated Molecular Weight: 848 aa, 96 kDa
• GenBank Accession Number: BC070080
• Gene Symbol: AHR
• Gene ID (NCBI): 196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)