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AHR Recombinant antibody, PBS Only

AHR Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240325G8

Cat no : 83369-5-PBS

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AHR Recombinant antibody (83369-5-RR)

    WB analysis using 83369-5-RR (same clone as 83369-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83369-5-RR (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83369-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using AHR Recombinant antibody (83369-5-RR)

    IHC staining of human ovary cancer using 83369-5-RR (same clone as 83369-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83369-5-RR (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83369-5-PBS in a different storage buffer formulation.

Product Information

The immunogen of 83369-5-PBS is AHR Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AHR fusion protein Ag12193
Full Name aryl hydrocarbon receptor
Calculated Molecular Weight 848 aa, 96 kDa
Observed Molecular Weight105-110 kDa
GenBank Accession NumberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)