AHR Recombinant antibody, PBS Only

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  WB analysis using 83369-5-RR (same clone as 83369-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83369-5-RR (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83369-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83369-5-RR (same clone as 83369-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83369-5-RR (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83369-5-PBS in a different storage buffer formulation.

AHR Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83369-5-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G8

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Product Information

83369-5-PBS targets AHR in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AHR fusion protein Ag12193
• Full Name: aryl hydrocarbon receptor
• Calculated Molecular Weight: 848 aa, 96 kDa
• Observed Molecular Weight: 105-110 kDa
• GenBank Accession Number: BC070080
• Gene Symbol: AHR
• Gene ID (NCBI): 196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P35869
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)