AHR Polyclonal antibody, PBS Only

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  WB analysis using 30242-1-AP (same clone as 30242-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30242-1-AP (AHR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30242-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 30242-1-AP (same clone as 30242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30242-1-AP (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30242-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 30242-1-AP (same clone as 30242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30242-1-AP (AHR antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30242-1-PBS in a different storage buffer formulation.

AHR Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 30242-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor

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Product Information

30242-1-PBS targets AHR in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHR fusion protein Ag32335
• Full Name: aryl hydrocarbon receptor
• Calculated Molecular Weight: 848 aa, 96 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC070080
• Gene Symbol: AHR
• Gene ID (NCBI): 196
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35869
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)