AHNAK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

AHNAK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 17682-1-AP

Synonyms

AHNAK nucleoprotein 2, AHNAK2, C14orf78, KIAA2019, Protein AHNAK2

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Validation Data Gallery

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  WB analysis using 17682-1-AP

  Various lysates were subjected to Tris-acetate gel system followed by western blot with 17682-1-AP (AHNAK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17682-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17682-1-AP (AHNAK2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 17682-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 17682-1-AP (AHNAK2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IHC detected in: human renal cell carcinoma tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 7 publications below
• IF: See 4 publications below

Product Information

17682-1-AP targets AHNAK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHNAK2 fusion protein Ag11779
• Full Name: AHNAK nucleoprotein 2
• Calculated Molecular Weight: 617 kDa
• GenBank Accession Number: BC049216
• Gene Symbol: AHNAK2
• Gene ID (NCBI): 113146
• RRID: AB_2878428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Int Immunopharmacol; AHNAK2 is a novel prognostic marker and correlates with immune infiltration in papillary thyroid cancer: Evidence from integrated analysis.; Authors - Long Zheng
human	IHC	Int Immunopharmacol; Correlation between prognostic indicator AHNAK2 and immune infiltrates in lung adenocarcinoma.; Authors - Miaosen Zheng
human	IHC,IF	Clin Exp Immunol; The AHNAK induces increased IL-6 production in CD4+T cells and serves as a potential diagnostic biomarker for recurrent pregnancy loss.; Authors - Liman Li
human	WB	Onco Targets Ther; AHNAK2 Promotes Migration, Invasion, and Epithelial-Mesenchymal Transition in Lung Adenocarcinoma Cells via the TGF-β/Smad3 Pathway.; Authors - Gang Liu ; KD Validated
human,mouse	WB,IHC,IF	Cancer Manag Res; AHNAK2 Promotes the Progression of Pancreatic Ductal Adenocarcinoma by Maintaining the Stability of c-MET; Authors - Zhaohui Chen
human	IHC	FEBS Open Bio; Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein-Barr virus-associated gastric cancer.; Authors - Hirofumi Ohmura