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AHCY Polyclonal antibody
AHCY Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 10757-2-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COLO 320 cells, HepG2 cells, mouse liver tissue, HeLa cells, Jurkat cells, rat liver tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 20 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Product Information
10757-2-AP targets AHCY in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, branchiostoma belcheri |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AHCY fusion protein Ag1208 |
Full Name | S-adenosylhomocysteine hydrolase |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 45-48 kDa |
GenBank Accession Number | BC011606 |
Gene Symbol | AHCY |
Gene ID (NCBI) | 191 |
RRID | AB_2289488 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).
Protocols
Product Specific Protocols | |
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WB protocol for AHCY antibody 10757-2-AP | Download protocol |
IHC protocol for AHCY antibody 10757-2-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Stem Cell Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal. | ||
Nat Commun H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase.
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Autophagy AHCYL1 senses SAH to inhibit autophagy through interaction with PIK3C3 in an MTORC1-independent manner.
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Clin Cancer Res Digital transcript profile analysis with aRNA-LongSAGE validates FERMT1 as a potential novel prognostic marker for colon cancer. | ||
Arterioscler Thromb Vasc Biol Increased plasma S-adenosylhomocysteine-accelerated atherosclerosis is associated with epigenetic regulation of endoplasmic reticulum stress in apoE-/- mice.
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Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kamal (Verified Customer) (01-26-2024) | Antibody worked well for western blot analysis when diluted with 1X TBST at the provided dilution.
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