|Positive WB detected in||pig heart tissue, pig skeletal muscle tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
66415-1-Ig targets AGTR1 in WB, ELISA applications and shows reactivity with human, rat, pig, mouse samples.
|Tested Reactivity||human, rat, pig, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||AGTR1 fusion protein Ag14461|
|Full Name||angiotensin II receptor, type 1|
|Calculated molecular weight||359 aa, 41 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC022447|
|Gene ID (NCBI)||185|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named as AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anca (Verified Customer) (10-30-2019)
We could detect AT1R from Plazenta, but only by using Chemiluminiscence. Unfortunately, the pozitive control that we used didn't worked.