CCP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 14067-1-AP

Synonyms

AGTPBP1, KIAA1035, EC:3.4.17.-, Cytosolic carboxypeptidase 1, ATP/GTP-binding protein 1

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Validation Data Gallery

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  WB analysis of mouse brain using 14067-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14067-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14067-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14067-1-AP (CCP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14067-1-AP

  IP result of anti-CCP1 (IP:14067-1-AP, 3ug; Detection:14067-1-AP 1:1000) with mouse testis tissue lysate 10000ug.

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  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14067-1-AP (CCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14067-1-AP (CCP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14067-1-AP (CCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 14067-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 14067-1-AP (CCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human testis tissue, human heart tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Product Information

14067-1-AP targets CCP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCP1 fusion protein Ag5122
• Full Name: ATP/GTP binding protein 1
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 138 kDa
• GenBank Accession Number: BC060815
• Gene Symbol: CCP1
• Gene ID (NCBI): 23287
• RRID: AB_2225559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.

Publications

Species	Application	Title
mouse	IF	Cell; Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis.; Authors - Carla G Silva
mouse	WB	Nat Commun; Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.; Authors - Buqing Ye ; KO Validated
human,mouse	WB,IF	EMBO J; Loss of tubulin deglutamylase CCP1 causes infantile-onset neurodegeneration.; Authors - Vandana Shashi
mouse	WB	Nat Commun; AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation.; Authors - Albert Herms
rat	WB	Proc Natl Acad Sci U S A; Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.; Authors - Maria Elena Pero ; KO Validated
human,mouse	WB,IP	J Cell Biol; CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice.; Authors - Stephen Gilmore-Hall