Angiotensinogen/AGT Monoclonal antibody, PBS Only

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  WB analysis of human plasma using 68020-1-Ig (same clone as 68020-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 68020-1-Ig (same clone as 68020-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig plasma using 68020-1-Ig (same clone as 68020-1-PBS)

  pig plasma were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 68020-1-Ig (same clone as 68020-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 68020-1-Ig (same clone as 68020-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 68020-1-Ig (AGT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68020-1-Ig (AGT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68020-1-Ig (same clone as 68020-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AGT antibody (68020-1-Ig, Clone: 1B2F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68020-1-PBS in a different storage buffer formulation.

Angiotensinogen/AGT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68020-1-PBS

Clone No.1B2F5

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

AGT, Angiotensinogen, Serpin A8, SERPINA8, 1B2F5

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Product Information

68020-1-PBS targets Angiotensinogen/AGT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Angiotensinogen/AGT fusion protein Ag16658
• Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC011519
• Gene Symbol: AGT
• Gene ID (NCBI): 183
• RRID: AB_2918765
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01019
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.