Angiotensinogen Polyclonal antibody

Angiotensinogen Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23972-1-AP

Synonyms

AGT, Serpin A8, SERPINA8, Ang IV, Ang III

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Validation Data Gallery

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  WB analysis using 23972-1-AP

  Human plasma were subjected to SDS PAGE followed by western blot with 23972-1-AP (Angiotensinogen antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 23972-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23972-1-AP (Angiotensinogen antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23972-1-AP

  Immunofluorescent analysis of HepG2 cells using 23972-1-AP (Angiotensinogen antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of HEK-293 using 23972-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Angiotensinogen antibody (23972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of L02 using 23972-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Angiotensinogen antibody (23972-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 23972-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Angiotensinogen (23972-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, HepG2 cells, L02 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

23972-1-AP targets Angiotensinogen in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Angiotensinogen fusion protein Ag21121
• Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC011519
• Gene Symbol: AGT
• Gene ID (NCBI): 183
• RRID: AB_2879383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Publications

Species	Application	Title
mouse	IF	JCI Insight; Osteopontin deficiency ameliorates Alport pathology by preventing tubular metabolic deficits.; Authors - Wen Ding