Angiotensinogen Polyclonal antibody

• Featured Product
• KD/KO Validated

Angiotensinogen Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 11992-1-AP

Synonyms

AGT, Serpin A8, SERPINA8, Ang IV, Ang III

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Validation Data Gallery

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  WB analysis using 11992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11992-1-AP (Angiotensinogen antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11992-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11992-1-AP (Angiotensinogen antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 11992-1-AP

  IP result of anti-Angiotensinogen (IP:11992-1-AP, 4ug; Detection:11992-1-AP 1:1000) with human plasma lysate 1600 ug.

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  IHC staining of human liver using 11992-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11992-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 11992-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11992-1-AP (Angiotensinogen antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11992-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Angiotensinogen antibody (11992-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HepG2 using 11992-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Angiotensinogen Polyclonal antibody (11992-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HuH-7 cells, HepG2 cells, human plasma
• Positive IP detected in: human plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Product Information

11992-1-AP targets Angiotensinogen in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Angiotensinogen fusion protein Ag2632
• Full Name: angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC011519
• Gene Symbol: AGT
• Gene ID (NCBI): 183
• RRID: AB_2225452
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensinogen is a precursor of angiotensin II (Ang II), is expressed and synthesized largely in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. It has a key role in mediating vascular constriction and regulating salt and fluid homeostasis. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene also have been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
mouse	IHC	Hypertension; Attenuation of brain damage and cognitive impairment by direct renin inhibition in mice with chronic cerebral hypoperfusion.; Authors - Dong Yi-Fei YF
mouse	WB	Biochem Pharmacol; Downregulation of spinal angiotensin converting enzyme 2 is involved in neuropathic pain associated with type 2 diabetes mellitus in mice.; Authors - Ryota Yamagata
mouse	IHC,IF	FASEB J; Sperm immotility is associated with epididymis metabolism disorder in mice under obstructive azoospermia; Authors - Sheng Gao
human	WB	Cells; Effect of Hydrocortisone on Angiotensinogen (AGT) Mutation-Causing Autosomal Recessive Renal Tubular Dysgenesis.; Authors - Min-Hua Tseng
mouse	WB	Phytomedicine; Epiberberine ameliorated diabetic nephropathy by inactivating the angiotensinogen (Agt) to repress TGFβ/Smad2 pathway.; Authors - Yaping Xiao