AGR3 Polyclonal antibody

AGR3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11967-1-AP

Synonyms

hAG 3, Anterior gradient protein 3, Anterior gradient 3 homolog, AG-3, AG3

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Validation Data Gallery

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  WB analysis of SKOV-3 using 11967-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11967-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11967-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11967-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11967-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11967-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 11967-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 11967-1-AP (AGR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

11967-1-AP targets AGR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGR3 fusion protein Ag2577
• Full Name: anterior gradient homolog 3 (Xenopus laevis)
• Calculated Molecular Weight: 166 aa, 19 kDa
• Observed Molecular Weight: 19-25 kDa
• GenBank Accession Number: BC058284
• Gene Symbol: AGR3
• Gene ID (NCBI): 155465
• RRID: AB_2877809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
chicken	IF	Elife; Single-cell proteomics reveals changes in expression during hair-cell development.; Authors - Ying Zhu
human	WB,IHC	Oncotarget; Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.; Authors - Soma Samanta
human	IHC, IF, WB	Front Pharmacol; AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD.; Authors - Rui Ye
chicken	IF	iScience; Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium; Authors - Mami Matsunaga
human	IF	J Transl Med; Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers; Authors - Youxue Zhang