AGPAT4 Polyclonal antibody

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  WB analysis using 16562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16562-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

AGPAT4 Polyclonal Antibody for WB, ELISA

Cat No. 16562-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

1-AGP acyltransferase 4, 1-acyl-sn-glycerol-3-phosphate acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1 AGPAT4, 1 AGPAT 4

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16562-1-AP targets AGPAT4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGPAT4 fusion protein Ag9834
• Full Name: 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
• Calculated Molecular Weight: 378 aa, 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC020209
• Gene Symbol: AGPAT4
• Gene ID (NCBI): 56895
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRZ5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGPAT4 (Acylglycerophosphate acyltransferase 4), also known as LPAATδ (Lysophosphatidic acid acyltransferase delta), is a protein expressed mainly in brain and in muscle. AGPAT4 is characterized as a mitochondrial lysophosphatidic acid acyltransferase that regulates brain levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) (PMID: 28807933). AGPAT4 is relatively abundant in murine renal white adipose tissue (WAT) and has been linked to the regulation of TAG production, the differentiation of adipocytes, and the modulation of various pathologies including lipodystrophy, diabetes, and hepatic steatosis (PMID: 28814640).