AGBL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

AGBL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 16990-1-AP

Synonyms

AGBL3, ATP/GTP binding protein like 3, CBPC3, CCP3

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Validation Data Gallery

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  WB analysis of mouse liver using 16990-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16990-1-AP (AGBL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 16990-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16990-1-AP (AGBL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 16990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 16990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16990-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16990-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, L02 cells
• Positive IHC detected in: human lung cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

16990-1-AP targets AGBL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGBL3 fusion protein Ag10592
• Full Name: ATP/GTP binding protein-like 3
• Calculated Molecular Weight: 73 kDa, 116 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC030651
• Gene Symbol: AGBL3
• Gene ID (NCBI): 340351
• RRID: AB_2878337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGBL3, also named as CCP3, is a metallocarboxypeptidase that may play a role in the processing of tubulin. AGBL3 has some isoforms with MW 107 kDA, 73 kDa, 116 kDa and 20 kDa.

Publications

Species	Application	Title
mouse	WB	J Exp Med; Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis.; Authors - Zhen Xiong ; KO Validated