human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14690-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14690-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 14690-1-AP
IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of human gliomas using 14690-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14690-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14690-1-AP
Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Hela cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14690-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 14690-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human gliomas using 14690-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 14690-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse brain using 14690-1-AP
IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IF/ICC Figures
IF Staining of HeLa using 14690-1-AP
Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
AGAP2 Polyclonal antibody
$149 (20ul) $449 (150ul)
Catalog Number
14690-1-AP
Citations
1
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:20-1:200
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
