AGA Recombinant antibody

AGA Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240396B4

Cat no : 83442-6-RR

Synonyms

Glycosylasparaginase alpha chain, GA, EC:3.5.1.26, ASRG, AGU

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Validation Data Gallery

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  WB analysis using 83442-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83442-6-RR (AGA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 83442-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83442-6-RR (AGA antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83442-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83442-6-RR against Human AGA were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells, mouse testis tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83442-6-RR targets AGA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AGA fusion protein Ag10681
• Full Name: aspartylglucosaminidase
• Calculated Molecular Weight: 346 aa, 37 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC012392
• Gene Symbol: AGA
• Gene ID (NCBI): 175
• RRID: AB_3671083
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGA(Aspartylglucosaminidase) is also named as N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase and glycosylasparaginase.The open reading frame of the readthrough transcript would indicate AGA close to 40 kDa. It can be cleaved into 2 chains:the 24 kDa α- and 17 kDa β-subunits of the wild-type AGA,in addition,an aberrant polypeptide with a mol.wt of 21 kDa is also detected as a product of proteolytic cleavage of the mutant precursor molecule(PMID: 9425233).AGA can exsit as a dimer with the mol.wt between 100 kDa and 49 kDa and His124 at an interface between two heterodimers of AGA is crucial for the thermodynamically stable oligomeric structure of AGA.(PMID:9737998). It can also exsit as a heterotetrameric protein of 88 kDa(PMID:1733831).