AFP Recombinant antibody

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  WB analysis of HepG2 using 82982-5-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 82982-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 82982-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFP antibody (82982-5-RR, Clone: 230435A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82982-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82982-5-RR against Human AFP were performed. The affinity constant is 0.696 nM.

AFP Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82982-5-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells, human placenta
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82982-5-RR targets AFP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: alpha-fetoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 68-72 kDa
• GenBank Accession Number: BC027881
• Gene Symbol: AFP
• Gene ID (NCBI): 174
• RRID: AB_3670731
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02771
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)