AES Polyclonal antibody

AES Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23913-1-AP

Synonyms

AES, AES 1, AES 2, Amino enhancer of split, ESP1, Gp130 associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5

---

Validation Data Gallery

• 

  WB analysis using 23913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23913-1-AP (AES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: DU 145 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23913-1-AP targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AES fusion protein Ag21021
• Full Name: amino-terminal enhancer of split
• Calculated Molecular Weight: 264 aa, 29 kDa
• Observed Molecular Weight: 22-29 kDa
• GenBank Accession Number: BC113735
• Gene Symbol: AES
• Gene ID (NCBI): 166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).