AEBP2 Polyclonal antibody

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  WB analysis of human testis using 11232-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11232-2-AP (AEBP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11232-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11232-2-AP (AEBP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11232-2-AP

  IP result of anti-AEBP2 (IP:11232-2-AP, 4ug; Detection:11232-2-AP 1:300) with mouse testis tissue lysate 4800ug.

AEBP2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 11232-2-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Synonyms

AE binding protein 2, AEBP2, Zinc finger protein AEBP2

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• ChIP: See 1 publications below

Product Information

11232-2-AP targets AEBP2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AEBP2 fusion protein Ag1756
• Full Name: AE binding protein 2
• Calculated Molecular Weight: 33 kDa, 54 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC015624
• Gene Symbol: AEBP2
• Gene ID (NCBI): 121536
• RRID: AB_2225962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZN18
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AEBP2, also named as Zinc finger protein AEBP2 or Adipocyte enhancer-binding protein 2, is a 517 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the AEBP2/jing C2H2-type zinc-finger family. AEBP2 as a DNA-binding transcriptional repressor may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. It may e playing a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract.

Publications

Species	Application	Title
mouse	WB	Nature; Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells.; Authors - Wu Hao H
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human		PLoS Genet; Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks.; Authors - Holdt Lesca M LM
mouse	WB,ChIP	Nucleic Acids Res; AEBP2 as a potential targeting protein for Polycomb Repression Complex PRC2.; Authors - Kim Hana H
mouse	WB	PLoS One; Aebp2 as an epigenetic regulator for neural crest cells.; Authors - Kim Hana H