Perilipin-2 Monoclonal antibody, PBS Only

Perilipin-2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5D4

Cat no : 60340-1-PBS

Synonyms

ADRP/Perilipin-2, ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2

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Validation Data Gallery

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  WB analysis of human adipose using 60340-1-Ig (same clone as 60340-1-PBS)

  human adipose tissue were subjected to SDS PAGE followed by western blot with 60340-1-Ig (ADRP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brown adipose using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brown adipose using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brown adipose using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

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Product Information

60340-1-PBS targets Perilipin-2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Perilipin-2 fusion protein Ag7643
• Full Name: adipose differentiation-related protein
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 47-52 kDa
• GenBank Accession Number: BC005127
• Gene Symbol: Perilipin-2
• Gene ID (NCBI): 123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.