ADRP Recombinant antibody, PBS Only

ADRP Recombinant Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230274F4

Cat no : 80362-2-PBS

Synonyms

ADFP, Adipophilin, ADRP, Perilipin 2, PLIN2

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Validation Data Gallery

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  WB analysis using 80362-2-RR (same clone as 80362-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 80362-2-RR (same clone as 80362-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 80362-2-RR (same clone as 80362-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 80362-2-RR (same clone as 80362-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80362-2-RR (same clone as 80362-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

• 

  Affinity experiments of 80362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80362-2-RR against Human ADRP were performed. The affinity constant is below 1 pM.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80362-2-PBS targets ADRP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ADRP fusion protein Ag7539
• Full Name: adipose differentiation-related protein
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC005127
• Gene Symbol: ADFP
• Gene ID (NCBI): 123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.