Perilipin-2 Recombinant antibody, PBS Only

Perilipin-2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230274F4

Cat no : 80362-2-PBS

Synonyms

ADRP/Perilipin 2, ADRP, ADFP, Perilipin 2, 230274F4

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Validation Data Gallery

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  WB analysis using 80362-2-RR (same clone as 80362-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  WB analysis using 80362-2-RR (same clone as 80362-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80362-2-RR (ADRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 80362-2-RR (same clone as 80362-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 80362-2-RR (same clone as 80362-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 80362-2-RR (ADRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80362-2-RR (same clone as 80362-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80362-2-RR (same clone as 80362-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 80362-2-RR (same clone as 80362-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using ADRP antibody (80362-2-RR, Clone: 230274F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 80362-2-RR (same clone as 80362-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ADRP Recombinant antibody (80362-2-RR, Clone:230274F4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80362-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80362-2-RR

  Biolayer interferometry (BLl) kinetic assays of 80362-2-RR against Human ADRP were performed. The affinity constant is below 1 pM.

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Product Information

80362-2-PBS targets Perilipin-2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Perilipin-2 fusion protein Ag7539
• Full Name: adipose differentiation-related protein
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC005127
• Gene Symbol: Perilipin-2
• Gene ID (NCBI): 123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.