ADRM1 Monoclonal antibody

ADRM1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A3

Cat no : 66464-1-Ig

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

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Validation Data Gallery

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  WB analysis using 66464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66464-1-Ig (ADRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66464-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ADRM1 antibody (66464-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, Raji cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66464-1-Ig targets ADRM1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADRM1 fusion protein Ag24699
• Full Name: adhesion regulating molecule 1
• Calculated Molecular Weight: 407 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC017245
• Gene Symbol: ADRM1
• Gene ID (NCBI): 11047
• RRID: AB_2881832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.