ADRBK1 Monoclonal antibody, PBS Only

ADRBK1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F7A5

Cat no : 67067-1-PBS

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, FLJ16718, GRK2

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Validation Data Gallery

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  WB analysis using 67067-1-Ig (same clone as 67067-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 67067-1-Ig (same clone as 67067-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67067-1-Ig (ADRBK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67067-1-Ig (ADRBK1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67067-1-Ig (same clone as 67067-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ADRBK1 antibody (67067-1-Ig, Clone: 2F7A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67067-1-Ig (same clone as 67067-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ADRBK1 (67067-1-Ig, Clone:2F7A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67067-1-PBS in a different storage buffer formulation.

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Product Information

67067-1-PBS targets ADRBK1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADRBK1 fusion protein Ag5071
• Full Name: adrenergic, beta, receptor kinase 1
• Calculated Molecular Weight: 689 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC037963
• Gene Symbol: ADRBK1
• Gene ID (NCBI): 156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.