GRK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

GRK2 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (2)

Cat no : 13990-1-AP

Synonyms

ARBK1, ADRBK1, BETA ARK1, Beta ARK 1, BARK1

---

Validation Data Gallery

• 

  WB analysis using 13990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13990-1-AP (GRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 13990-1-AP

  IP result of anti-GRK2 (IP:13990-1-AP, 4ug; Detection:13990-1-AP 1:1000) with HL-60 cells lysate 3040ug.

• 

  IHC staining of human lymphoma using 13990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 13990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13990-1-AP (GRK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 13990-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug GRK2 Polyclonal antibody (13990-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HL-60 cells, Jurkat cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13990-1-AP targets GRK2 in WB, IHC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GRK2 fusion protein Ag5071
• Full Name: adrenergic, beta, receptor kinase 1
• Calculated Molecular Weight: 689 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC037963
• Gene Symbol: ADRBK1
• Gene ID (NCBI): 156
• RRID: AB_2225833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)

Publications

Species	Application	Title
human	WB	Nat Commun; Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3SPOP E3 ligase in cancer cells.; Authors - Qiyin Zhou
human	WB	Nat Commun; Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.; Authors - Fan Yang ; KD Validated
human,mouse	WB,CoIP	J Pharm Anal; Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis; Authors - Jia-Jia Zhang ; KD Validated
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	J Am Heart Assoc; Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension.; Authors - Xuejing Sun ; KD Validated
human	WB	iScience; 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone provokes progression from chronic pancreatitis to pancreatic intraepithelial neoplasia.; Authors - Xin Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Charlotte (Verified Customer) (07-23-2024)

I tested the antibody as followed: Blocking 1h in 5% milk in TBST 1/1000 in blocking solution, O.N. at 4°C 5*5min TBST 1/5000 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS in IMCD3 ; I obtained GRK2 signal (the middle band out of the 3) but also many aspecific bands. I also tested it in IF since I had it (the ab is not approved for this application) but I got no signal

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: IMCD3

GRK2 Antibody Western Blot,Immunofluorescence validation (1/1000 dilution) in IMCD3 (Cat no:13990-1-AP)

FH

Franziska (Verified Customer) (09-11-2023)

Since there are no GRK-2 antibodies specific for C.elegans, I decided to try this one which didn't work very well, there was a lot of unspecific binding however, C.elegans was not mentioned as tested reactivity.

• Applications: Western Blot
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: C.elegans

GRK2 Antibody Western Blot validation (1:750 dilution) in C.elegans (Cat no:13990-1-AP)