ADRB2 Polyclonal antibody

ADRB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 29864-1-AP

Synonyms

Beta-2 adrenergic receptor, Beta 2 adrenergic receptor, BAR, B2AR, ADRBR

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Validation Data Gallery

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  WB analysis using 29864-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 29864-1-AP (ADRB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29864-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 29864-1-AP (ADRB2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

29864-1-AP targets ADRB2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADRB2 fusion protein Ag32313
• Full Name: adrenergic, beta-2-, receptor, surface
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46-48 kDa
• GenBank Accession Number: BC012481
• Gene Symbol: ADRB2
• Gene ID (NCBI): 154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity, and type 2 diabetes. This antibody detects ADRB2 with a molecular weight of 46-48 kDa as has been shown by some researches (PMID: 30542705; 35075132).

Publications

Species	Application	Title
human,mouse	WB	iScience; Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration; Authors - Hao-Kun Xu
human	WB	Oncol Lett; β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways; Authors - Xingcheng Lin
mouse	WB	Immunobiology; Exploring liquid-liquid phase separation-related diagnostic biomarkers in osteoarthritis based on machine learning algorithms and experiment; Authors - Bo Dong
human,mouse	IHC	Adv Sci (Weinh); Chronic Stress-Induced and Tumor Derived SP1+ Exosomes Polarizing IL-1β+ Neutrophils to Increase Lung Metastasis of Breast Cancer; Authors - Leyi Zhang