ADRB1 Polyclonal antibody

ADRB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28323-1-AP

Synonyms

ADRB1, ADRB1R, adrenergic, beta 1 , receptor, B1AR, Beta 1 adrenergic receptor, Beta 1 adrenoceptor, Beta 1 adrenoreceptor, BETA1AR, RHR



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

28323-1-AP targets ADRB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRB1 fusion protein Ag28223
Full Name adrenergic, beta-1-, receptor
Calculated Molecular Weight 51 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberNM_000684
Gene Symbol ADRB1
Gene ID (NCBI) 153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRB1 (adrenergic receptor beta 1), a member of the G protein-coupled receptor (GPCR) superfamily, responds to catecholamine stimulation. ADRB1 is the predominant subtype expressed in the heart and mediates increases in inotropy and chronotropy when stimulated. ADRB1 mediates a wide range of cardiovascular physiological responses and has been of great interest to researchers as a therapeutic target for cardiovascular diseases. Moreover, three subtypes of ADRBs (ADRB1, ADRB2, and ADRB3) are encoded by three separate genes (PMID:16636683; 33372534; 33593484).

Protocols

Product Specific Protocols
WB protocol for ADRB1 antibody 28323-1-APDownload protocol
IHC protocol for ADRB1 antibody 28323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

iScience

Region-specific sympatho-adrenergic regulation of specialized vasculature in bone homeostasis and regeneration

Authors - Hao-Kun Xu
humanWB

Oncol Lett

β‑adrenergic receptor activation promotes the proliferation of HepG2 cells via the ERK1/2/CREB pathways

Authors - Xingcheng Lin
ratWB

Brain Res

The α1 adrenoceptor antagonist prazosin potentiates morphine induced conditioned place preference in rats

Authors - Wanyu Tu