ADRA1B-Specific Polyclonal antibody

ADRA1B-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (2)

Cat no : 19776-1-AP

Synonyms

ADRA1, ADRA1B, ADRA1B-Specific, Alpha 1B adrenergic receptor, Alpha 1B adrenoceptor, Alpha 1B adrenoreceptor, ALPHA1BAR

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Validation Data Gallery

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  IHC staining of mouse brain using 19776-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19776-1-AP (ADRA1B-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19776-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19776-1-AP (ADRA1B-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 19776-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19776-1-AP (ADRA1B-Specific Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 19776-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19776-1-AP (ADRA1B-Specific Antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse brain tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

19776-1-AP targets ADRA1B-Specific in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: adrenergic, alpha-1B-, receptor
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: NM_000679
• Gene Symbol: ADRA1B
• Gene ID (NCBI): 147
• RRID: AB_2848149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRA1B belongs to the G-protein coupled receptor 1 family. ADRA1B mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This antibody is specific to ADRA1B.

Publications

Species	Application	Title
mouse	IF	J Comp Neurol; Structural basis for noradrenergic regulation of neural circuits in the mouse olfactory bulb.; Authors - Sawa Horie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Andrea (Verified Customer) (07-31-2023)

Good signal in flow cytometry. Endogenously expressed protein and confirmed with overexpressing cell line

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1ug/ml

FH

Mohamad (Verified Customer) (07-03-2023)

different bands at different MW

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Lung