ADNP Polyclonal antibody

ADNP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17987-1-AP

Synonyms

KIAA0784, ADNP1, Activity-dependent neuroprotector homeobox protein, Activity-dependent neuroprotective protein

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Validation Data Gallery

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  WB analysis using 17987-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17987-1-AP (ADNP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17987-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 17987-1-AP (ADNP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 17987-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17987-1-AP (ADNP antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 17987-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADNP antibody (17987-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

17987-1-AP targets ADNP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADNP fusion protein Ag12414
• Full Name: activity-dependent neuroprotector homeobox
• Calculated Molecular Weight: 1102 aa, 123 kDa
• Observed Molecular Weight: 135-145 kDa
• GenBank Accession Number: BC075794
• Gene Symbol: ADNP
• Gene ID (NCBI): 23394
• RRID: AB_2222383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; ADNP Upregulation Promotes Bladder Cancer Cell Proliferation via the AKT Pathway.; Authors - Shuai Zhu
mouse	IHC	J Cancer; ADNP prompts the cisplatin-resistance of bladder cancer via TGF-β-mediated epithelial-mesenchymal transition (EMT) pathway.; Authors - Yu Xie
human	IF	Mol Autism; Clinical impact and in vitro characterization of ADNP variants in pediatric patients; Authors - Chuanhui Ge