Adiponectin Recombinant antibody, PBS Only (Capture)

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  IF Staining of 3T3-L1 using 83961-1-RR (same clone as 83961-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using Adiponectin antibody (83961-1-RR, Clone: 241082C11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83961-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00919-1

  Cytometric bead array standard curve of MP00919-1, Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83961-1-PBS. Detection antibody: 83961-2-PBS. Standard: Ag16304. Range: 1.25-80 ng/mL.

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  Cytometric bead array standard curve of MP00919-2

  Cytometric bead array standard curve of MP00919-2, Adiponectin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83961-1-PBS. Detection antibody: 83961-3-PBS. Standard: Ag16304. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 83961-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83961-1-RR against Human Adiponectin were performed. The affinity constant is below 1 pM.

Adiponectin Uni-rAb^TM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 83961-1-PBS

Clone No.241082C11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

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Product Information

83961-1-PBS targets Adiponectin as part of a matched antibody pair:

MP00919-1: 83961-1-PBS capture and 83961-2-PBS detection (validated in Cytometric bead array)

MP00919-2: 83961-1-PBS capture and 83961-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Adiponectin fusion protein Ag16304
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 244 aa, 26 kDa
• GenBank Accession Number: BC096308
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 9370
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15848
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.