ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3D12E9

Cat no : 67303-1-Ig

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

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Validation Data Gallery

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  WB analysis using 67303-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67303-1-Ig (ADH4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 67303-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ADH4 antibody (67303-1-Ig, Clone: 3D12E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, HuH-7 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67303-1-Ig targets ADH4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ADH4 fusion protein Ag9884
• Full Name: alcohol dehydrogenase 4 (class II), pi polypeptide
• Calculated Molecular Weight: 380 aa, 40 kDa
• Observed Molecular Weight: 40-43 kDa
• GenBank Accession Number: BC022319
• Gene Symbol: ADH4
• Gene ID (NCBI): 127
• RRID: AB_2882565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.